Table 4. Ordering of the candidate reference genes according to their expression stability values calculated by BestKeeper.
Pods | Tissues | 4°C | 40°C | NaCl | PEG | 4°C and 40°C | NaCl and PEG | All samples | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Genes | Values | Genes | Values | Genes | Values | Genes | Values | Genes | Values | Genes | Values | Genes | Values | Genes | Values | Genes | Values |
ACT | 1.00 | ACP2 | 0.97 | PEPC | 0.95 | TUB | 1.00 | MDH2 | 0.90 | ACP2 | 0.99 | TUA1 | 0.98 | ACP2 | 0.92 | UEP | 0.97 |
UEP | 0.99 | TUA1 | 0.97 | ACP1 | 0.89 | TUA1 | 0.98 | ACP2 | 0.90 | EF2 | 0.98 | UEP | 0.95 | RPL1 | 0.87 | ACP2 | 0.97 |
CYP2 | 0.98 | TUB | 0.94 | MDH2 | 0.88 | EF2 | 0.96 | ACP1 | 0.84 | MDH2 | 0.97 | EF2 | 0.95 | UEP | 0.86 | RPL1 | 0.96 |
MDH2 | 0.97 | TUA2 | 0.93 | TUA2 | 0.84 | MDH1 | 0.96 | MDH1 | 0.78 | MDH1 | 0.94 | MDH1 | 0.94 | MDH2 | 0.79 | ACT | 0.95 |
ACP2 | 0.97 | RPL1 | 0.93 | TUB | 0.69 | UEP | 0.95 | CYP1 | 0.78 | ACP1 | 0.90 | RPL1 | 0.93 | EF2 | 0.77 | ACP1 | 0.94 |
RPL1 | 0.95 | ACT | 0.91 | ACP2 | 0.56 | RPL2 | 0.92 | RPL2 | 0.73 | UEP | 0.89 | ACP1 | 0.91 | RPL2 | 0.74 | MDH2 | 0.89 |
EF1 | 0.92 | EF1 | 0.83 | RPL1 | 0.50 | RPL1 | 0.90 | EF2 | 0.65 | RPL2 | 0.88 | RPL2 | 0.86 | ACP1 | 0.70 | EF1 | 0.84 |
EF2 | 0.91 | CYP1 | 0.83 | ACT | 0.43 | ACP1 | 0.90 | RPL1 | 0.14 | RPL1 | 0.83 | ACP2 | 0.84 | GADPH | 0.60 | GADPH | 0.72 |
RPL2 | 0.72 | UEP | 0.83 | MDH1 | 0.41 | CYP1 | 0.87 | ACT | -0.36 | GADPH | 0.60 | CYP1 | 0.81 | ACT | 0.21 | CYP1 | 0.69 |
ACP1 | 0.66 | ACP1 | 0.80 | CYP2 | 0.33 | ACP2 | 0.83 | UEP | -0.50 | ACT | -0.13 | ACT | 0.79 | EF1 | 0.13 | RPL2 | 0.67 |