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. 2016 Aug 19;12(8):e1006270. doi: 10.1371/journal.pgen.1006270

Fig 5. Localization of O10 in the PB and the Interactions between O10 and different type of zeins.

Fig 5

(A) Immunoblotting of O10 in the fraction of PB that was treated with Triton X-100. S, supernatant (the surrounding ER); P, pellet (the PB core). The fraction without Triton X-100 treatment was used as a negative control. Fractions were subjected to immunoblot analysis with antibodies against the N terminus of O10 (anti-O10), the C terminus of O10 (anti-O10-C), BIP (ER marker), or 22-kD α-zein (PB marker). (B) Immunolocalization of O10 in wild-type endosperm samples (21 DAP). The red arrowheads indicate O10 gold labeling on the PB; Bars = 0.5 μm. (C) O10-N represents the N terminus of O10 from 1–268 aa. The interaction between T-antigen and Human P53 was used as a positive control. The interaction between pGBKT7-O10-N and pGADT7-empty was used as a negative control. AD, activating domain; BD, binding domain. (D) LCI assay showing that the N terminus of O10 interacts with different types of zeins. Fluorescence signal intensities represent their interaction activities.