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. 2016 Aug 19;12(8):e1006270. doi: 10.1371/journal.pgen.1006270

Fig 7. The interactions of O10 with O1, HIP and FL1 and the accumulation of O10 in other opaque mutants.

Fig 7

(A) Yeast two-hybrid interactions between the O10-N and O1-Tail, HIP and FL1. The interaction between T-antigen and Human P53 was used as a positive control. The interaction between pGBKT7-O10-N and pGADT7-empty was used as a negative control. AD, activating domain; BD, binding domain. (B) LCI showing that O10 interacts with HIP and FL1. Fluorescence signal intensities represent the interaction activities. (C) Immunoblot analysis of O10 and the accumulation of different zein proteins in different opaque mutants and corresponding wild-type mature kernels. Anti-tubulin was used as a sample loading control.