Fig 4. Enriched motifs for RNA binding proteins in exons differentially spliced in photoreceptors and expression of neuronal splicing regulators in the retina.

(A) Diagram showing the position and enrichment of RNA binding protein motifs in proximity to the exons that are differentially spliced in photoreceptors. Sites enriched/depleted in the upregulated exons are shown on top in green and sites enriched/depleted in downregulated exons are shown below the exon diagram in red. Stacked ovals indicate clusters of binding sites. Pale colors indicate depletion of the motif. The fold enrichment and false discovery rate corrected p-values are shown next to each site. Clustered motifs are also labeled with the minimum number of motifs per cluster and the maximum spacing between the motifs in the cluster. (B) Immunofluorescence (IF) staining of retinal sections with antibodies against RBFOX, NOVA1, ELAVL, PTBP1, PTBP2, MSI1, MSI2, KHDRBS1, KHDRBS2 and KHDRBS3. IF signal is shown in grayscale and DAPI staining of the nuclear DNA is in blue. DAPI staining, shown for half of each section, indicates the position of the outer nuclear (ONL), inner nuclear (INL) and ganglion cell (GCL) layers.