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Optical properties of overexpressed Gaussia princeps luciferase: Gaussia princeps luciferase was overexpressed in a bacterial expression system after induction with IPTG and purified using affinity chromatography. Analysis of the emission spectrum displays the maximum intensity at 465 nm (A). Determination of total photon emission rate was performed in an IVIS-100 imaging system over a time period of 510 s (B).
