Figure 6.

GO terms analysis in S. pombe reveals that retained introns translation preference is not unique to genes with a very specific function. (A--–C) Summary of retained peptide length (A) and Z-score corresponding to peptide length relatively to randomized models (B) for biological process demonstrates that in 61% of the gene groups there is preference for a STOP codon closer to the 5′SS. (C) Statistical analysis indicates that the empirical P-value related to the observed signal is <0.1 in 22% of the examined gene groups (see Materials and methods). (D and E) Summary of average tAI (D) and Z-score corresponding to tAI levels relatively to randomized models (E; in absolute values) profiles in the pre-mRNA domains for biological process demonstrates that in various gene groups there is preference for higher tAI values at the beginning of introns in comparison to the region downstream from the first intronic STOP codon; statistical analysis indicates that the observed signal is significant in 71% of the examined gene groups (P <  0.05 for all cases, Wilcoxon rank-sum test; see Materials and methods). Thus, the reported signal is universal and not related to any one functional gene group.