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. 2016 Aug 17;4:e2345. doi: 10.7717/peerj.2345

Table 1. Primers of candidate genes and reference library constructing.

Only the c and h were used for high-throughput sequencing in fusion primer mode (primer + tags). The unique tags were used to differentiate PCR products pooled together for highthroughput sequencing (Parameswaran et al., 2007).

Gene Primer Sequence (5′-3′)
matK matK-XFa TAATTTACGATCAATTCATTC
matK-MALPb ACAAGAAAGTCGAAGTAT
rbcL rbcLa-Fc ATGTCACCACAAACAGAGACTAAAGC
rbcLa-Rd GTAAAATCAAGTCCACCRCG
trnH-psbA pasbA3_fe CGCGCATGGTGGATTCACAATCC
trnHf_05f GTTATGCATGAACGTAATGCTC
trnL cg CGAAATCGGTAGACGCTACG
hh CCATTGAGTCTCTGCACCTATC

Notes.

a

referred to Ford et al. (2009).

c

referred to Hasebe et al. (1994).

d

referred to Kress et al. (2009).

e

referred to Tate & Simpson (2003).

g

referred to Taberlet et al. (1991).

h

referred to Taberlet et al. (2007).