Table 1. Primers of candidate genes and reference library constructing.
Only the c and h were used for high-throughput sequencing in fusion primer mode (primer + tags). The unique tags were used to differentiate PCR products pooled together for highthroughput sequencing (Parameswaran et al., 2007).
Gene | Primer | Sequence (5′-3′) |
---|---|---|
matK | matK-XFa | TAATTTACGATCAATTCATTC |
matK-MALPb | ACAAGAAAGTCGAAGTAT | |
rbcL | rbcLa-Fc | ATGTCACCACAAACAGAGACTAAAGC |
rbcLa-Rd | GTAAAATCAAGTCCACCRCG | |
trnH-psbA | pasbA3_fe | CGCGCATGGTGGATTCACAATCC |
trnHf_05f | GTTATGCATGAACGTAATGCTC | |
trnL | cg | CGAAATCGGTAGACGCTACG |
hh | CCATTGAGTCTCTGCACCTATC |
Notes.
referred to Ford et al. (2009).
referred to Dunning & Savolainen (2010).
referred to Hasebe et al. (1994).
referred to Kress et al. (2009).
referred to Tate & Simpson (2003).
referred to Sang, Crawford & Stuessy (1997).
referred to Taberlet et al. (1991).
referred to Taberlet et al. (2007).