Table 1. Summary of analysis results for lung development transcriptional profiling in three inbred mouse strainse.
The cumulative variation in gene expression accounted for by the first 10 principal components is 80.9%. Pearson Correlation results are from the pairwise correlation of PCA scores as a function of strain. Regression results show the significance levels of the effect of three variables (developmental stage, strain, and stage by strain interaction) for each Principal Component. Tukey HSD connecting letters diagram indicates relatedness of strains for each component.
| Component | Var. (%) | Pearson Correlation (R2) | Regression (Pval) | Tukey HSD | |||
|---|---|---|---|---|---|---|---|
| PC1 | 55.1 (55.1) | C57BL/6J-A/J | .990*** | Stage | <.0001† | AJ | A |
| A/J-C3H/HeJ | .993*** | Strain | 0.9131 | B6 | A | ||
| C57BL/6J-C3H/HeJ | .990*** | Stage*strain | 0.6566 | C3H | A | ||
| PC2 | 6.1 (61.2) | C57BL/6J-A/J | .744*** | Stage | <.0001† | AJ | A |
| A/J-C3H/HeJ | .839*** | Strain | 0.5970 | B6 | A | ||
| C57BL/6J-C3H/HeJ | .849*** | Stage*strain | 0.0038 | C3H | A | ||
| PC3 | 4.98 (66.2) | C57BL/6J-A/J | .865*** | Stage | <.0001† | AJ | A |
| A/J-C3H/HeJ | .739*** | Strain | 0.2229 | B6 | A | ||
| C57BL/6J-C3H/HeJ | .646*** | Stage*strain | 0.2090 | C3H | A | ||
| PC4 | 3.53 (69.7) | C57BL/6J-A/J | .117 | Stage | <.0001† | AJ | B |
| A/J-C3H/HeJ | .114 | Strain | 0.0072 | B6 | B | ||
| C57BL/6J-C3H/HeJ | .192 | Stage*strain | 0.0029 | C3H | A | ||
| PC5 | 2.83 (72.5) | C57BL/6J-A/J | .379* | Stage | <.0001† | AJ | B |
| A/J-C3H/HeJ | .445** | Strain | <.0001† | B6 | C | ||
| C57BL/6J-C3H/HeJ | .262 | Stage*strain | <.0001† | C3H | A | ||
| PC6 | 2.27 (74.8) | C57BL/6J-A/J | .130 | Stage | <.0001† | AJ | B |
| A/J-C3H/HeJ | .231 | Strain | <.0001† | B6 | A | ||
| C57BL/6J-C3H/HeJ | .214 | Stage*strain | 0.0006† | C3H | B | ||
| PC7 | 2.02 (76.8) | C57BL/6J-A/J | .522*** | Stage | <.0001† | AJ | A |
| A/J-C3H/HeJ | .561*** | Strain | <.0001† | B6 | B | ||
| C57BL/6J-C3H/HeJ | .734*** | Stage*strain | 0.0385 | C3H | A | ||
| PC8 | 1.55 (78.4) | C57BL/6J-A/J | .286 | Stage | <.0001† | AJ | B |
| A/J-C3H/HeJ | .469*** | Strain | <.0001† | B6 | A | ||
| C57BL/6J-C3H/HeJ | .131 | Stage*strain | 0.0437 | C3H | A | ||
| PC9 | 1.43 (79.8) | C57BL/6J-A/J | .344* | Stage | <.0001† | AJ | B |
| A/J-C3H/HeJ | .479*** | Strain | <.0001† | B6 | C | ||
| C57BL/6J-C3H/HeJ | .194 | Stage*strain | <.0001† | C3H | A | ||
| PC10 | 1.08 (80.9) | C57BL/6J-A/J | .320 | Stage | <.0001† | AJ | C |
| A/J-C3H/HeJ | .386** | Strain | <.0001† | B6 | B | ||
| C57BL/6J-C3H/HeJ | .181 | Stage*strain | <.0001† | C3H | A | ||
Notes.
*
p-value < 0.01.
**
p-value < 0.001.
***
p-value < 0.0001.
†
Significant effect after multiple testing correction (FDR < 0.1).
The dataset used as input for the PCA and regression analyses is provided in Data S2.