Fig. 1.

(A) Schematic representation of the TREX-2 complex that is formed around a Sac3 scaffold (brown). The Sac3 scaffold protein can be broadly split into three sections whereby the most N-terminal region of Sac3 (labeled Sac3^N) and its homologues (such as GANP from H. sapiens) contain FG-nucleoporin-like motifs. The middle region of Sac3 (labeled Sac3^M) forms a complex with Thp1 and Sem1 to form a nucleic acid binding platform formed by its winged-helix domains, and the C-terminal region of Sac3 (labeled Sac3^CID) interacts with one Cdc31 chain (yellow) and two Sus1 chains (red) in S. cerevisiae. (B) Flowchart for the purification scheme established for producing recombinant TREX-2 complex and the corresponding gels. Numbers in red match the purification step and the Coomassie-stained SDS gel.