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. 2016 Aug 19;2(8):e1600379. doi: 10.1126/sciadv.1600379

Fig. 1. Expression constructs and protocol.

Fig. 1

(A) Schematic representation of the nascent chain constructs contained in the investigated stably arrested ribosome-nascent chain complexes (RNCs). After cleavage of the triple Strep-tag by Ubl-specific protease 1 (Ulp1), the remaining nascent chain consists of the signal sequence of DsbA and the SecM stalling sequence; the nascent chain sequences measured in this study are drawn together with their predicted secondary structure (C, coil; H, helical) and the confidence value of the prediction, as provided by PSIPRED (29). Serine residues are depicted in red. WT, wild type. (B) Expression scheme for the production of stably arrested RNCs. IPTG, isopropyl-β-d-thiogalactopyranoside.