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. 2016 Aug 6;5:e16078. doi: 10.7554/eLife.16078

Figure 5. YME1L1 mutation impairs fibroblast cell growth and proliferation.

Figure 5.

(A) Significant reduction of cell culture growth (y-axis scale *104) and (B) proliferation of patient primary human adult fibroblasts after 120h under culture conditions (Co, Control; II.5, patient II.5; one-way ANOVA; ***p<0.001; ****p<0.0001; n=5 ). (C) No increase in apoptosis-sensitivity upon 2 µM staurosporine (STS) treatment in patient HAF after 6h (SDS-PAGE for cleaved PARP level (cPARP) (Co, Control; II.5, patient II.5; one-way ANOVA; p6h = 0.4283; n=3; ). (D) Normal respiratory chain subunit activity in patient HAF (U, units; mU, milli U; CS, Citrate synthase). (E) Cytochrome c oxidase subunit 4 (COX4) and NADH ubiquinone oxidoreductase 1 beta subcomplex 6 (NDUFB6) protein levels were not elevated in patient fibroblasts (Co, Control; II.5, patient II.5; n=3). (F) Decreased levels of mitochondrial compartment markers TOMM20 (translocon of outer mitochondrial membrane 20) for the outer membrane (OM), Cytochrome c for the intermembrane space (IMS), SDHA (succinate dehydrogenase) for the inner membrane (IM), and Cyclophilin D for the matrix in whole cell lysates of patient II.5 (Co, Control; II.5, patient II.5; n=3).

DOI: http://dx.doi.org/10.7554/eLife.16078.015

Figure 5—source data 1. Raw data Graph 5A.
Raw values of fluorometric measurement with Cell-titer Blue Cell Viability Assay application in human adult fibroblasts of controls and patient (n=5).
DOI: 10.7554/eLife.16078.016
Figure 5—source data 2. Raw data Graph 5B.
Raw values of colorimetric measurement with BrdU Cell Proliferation ELISA Assay application in human adult fibroblasts of controls and patient (n=5).
DOI: 10.7554/eLife.16078.017
Figure 5—source data 3. Raw data Graph 5C.
Raw values of cleaved-PARP antibody signal intensity after staurosporine treatment in human adult fibroblasts of controls and patient and SDS-Page (n=3).
DOI: 10.7554/eLife.16078.018