Table 5.

Identification of proteins in 16 2-D gel spots significantly affected by maternal smoking in human term placenta. The proteins are grouped into two networks according to the Ingenuity Pathway Analysis (IPA) (see Supplementary Table 2).

Spot #	Gene symbol	Protein name	Accession #	Fold-change	p-valuea	MW	pI	MOWSE Score
Network 1: Cell Morphology, Cellular Assembly and Organization, Cellular Compromise
118	SERPINB2	Plasminogen activator inhibitor 2	NP_002566	−1.9	0.041	46.8	5.46	528
1828	SERPINA1	Alpha-1-antitrypsin	NP_000286	−1.5	0.035	46.9	5.37	974
2047b	SERPINA1	Alpha-1-antitrypsin (or S variant)	1QMB_A	+1.8	0.004	36.6	5.44	1086
1077	SERPINA1	Alpha-1-antitrypsin	AAH15642	+1.8	<0.001	39.1	5.27	942
2048b	SERPINA1	Alpha-1-antitrypsin	AAB59495	+1.6	0.016	46.8	5.43	716
1468b	PPIB	Peptidyl-prolyl cis-trans isomerase B	AAA52150	−1.5	0.008	22.8	9.33	309
1487b	PRDX1	Peroxiredoxin 1	NP_002565	−1.5	0.036	22.3	8.27	480
1468b	FGA	Fibrinogen alpha chain	0501249A	−1.5	0.008	26.6	9.08	444
1470	FGA	Fibrinogen alpha chain	0501249A	−1.7	0.002	26.6	9.08	425
2047b	VIM	Vimentin	AAA61279	+1.8	0.004	53.7	5.03	426
2048b	VIM	Vimentin	AAA61279	+1.6	0.016	53.7	5.03	724
1793	COL1A1	Collagen alpha 1(I) Chain	AAH36531	−1.5	0.005	139.9	5.70	187
2105d	COL1A1	Collagen alpha 1(I) Chain	AAH36531	−1.6	<0.001	140.0	5.70	125
1966	KRT8	KRT8 protein	AAH11373	+1.5	0.017	41.1	4.94	582
1107	KRT18	KRT18 protein, cytoskeletal	CAA31377	−1.5	<0.001	47.3	5.27	1337
Network 2: DNA Replication, Recombination, and Repair, Energy Production, Nucleic Acid Metabolism
1342	PNP	Purine nucleoside phosphorylase	NP_000261	−1.6	<0.001	32.3	6.45	684
1379	CA1	Carbonic anhydrase 1	NP_001729	−1.5	0.004	28.9	6.59	617
1468b	ATP5F1	ATP synthase subunit b, mitochondoral	AAH05960	−1.5	0.008	28.9	9.45	218
1487b	TAGLN2	Transgelin-2	NP_003555	−1.5	0.036	22.5	8.41	450
1969c	HBB	Hemoglobin subunit beta	2DXM_D	−2.6	0.032	15.9	6.55	643
1349	EFHD1	EF-hand domain-containing protein D1	NP_079479	+1.6	0.004	27.0	5.34	408

^aAs determined using log-normalized spot volumes.

^bMore than one protein highly likely to be in the spot.

^cPoor gel match with MW and pI but sequence coverage of 94% and an ion score of 21 suggests an accurate identification.

^dPoor sequence coverage and gel MW match.