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. Author manuscript; available in PMC: 2016 Aug 19.
Published in final edited form as: Biotechnol J. 2009 Jan;4(1):119–128. doi: 10.1002/biot.200800224

Table 2.

Samples used in the asymmetric PCR.

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Template 16A 16A 16B 16B Template(μL) 16A 16A 16B 16B
10mM dNTP 4 4 4 4 10mM dNTP 4 4 4 4
2U/μL Hot start 2.4 2.4 2.4 2.4 2U/μL Hot start 2.4 2.4 2.4 2.4
10× hot start 20 20 20 20 10× hot start 20 20 20 20
50μM Left L20 5 0 5 0 100μM 2.5 0 2.5 0
50μM Right R20 0 5 0 5 100μM Tag-R20 0 2.5 0 2.5
Nuclease-free 152.6 152.6 152.6 152.6 Nuclease-free 155.1 155.1 155.1 155.1