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. 2016 Aug 17;7:12535. doi: 10.1038/ncomms12535

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Flow cytometry histogram shows reprogrammed hybridoma cells (PnP-HEL23) surface express antibody specific for cognate antigen hen egg lysozyme (HEL). (b) ELISA data show that PnP-HEL23 cells secrete antibody specific for HEL. One technical replicate was analysed for the control samples (WT and PnP-mRuby) and two replicates for the PnP-HEL23 cell line. A four-parameter logistical curve was fitted to the data by nonlinear regression. Data are presented as the mean and error bars indicate standard deviation. Throughout this figure, WT cells correspond to clone WEN1.3, PnP-mRuby cells correspond to clone 1E9.C3, PnP-HEL23 cells correspond to clone Y (for more details, see Supplementary Table 1).