Table 1. Oligonucleotide sequences and quantitative PCR conditions.
| Cluster | Reference sequence | Forward primer 5′–3′ | Reverse primer 5′–3′ | Probe | Annealing temperature (°C) |
|---|---|---|---|---|---|
| P1 | M773_3_70_4 | GGACTACATTCGGACTAG | GTCGTAACCACGATCTAG | TCTTCAAAATCCCGCGTCCCG (antisense) | 60 |
| P2 | M773_3_150_7 | GGTGTTCTATGTGTTGAA | GTAGGAGTTACGAATTGG | TCGCCTAGCACATCATAGATCAC (antisense) | 50 |
| P3 | M773_56_100_1 | CACAGTTAGAGAGGTAGG | CAAGGTCGTCAGTAAAAG | AGCTCGACAAGGTAATGTTCACA (sense) | 54 |
| P4 | M773_22_30_1 | CTCGCACAGAAATCAGTG | GCATGTTAATGGAAGTGATG | ACGTCGAACTCGAAGACATCCG (sense) | 60 |
| P5 | M773_56_100_11 | GGAAGTCTTACTTGAAGG | CACCATTTTCCTCAAGAA | ATTGCTGTAATAACGCCTCTGC (antisense) | 60 |
| P6 | M773_56_85_3 | GCTCAATCTACAATTATGC | GCTGTAATAACTCCTCTAC | ACCACCTGACTCAGTACAATTAATGT (antisense) | 50 |
| P7 | M773_28_115_1 | GGTTCTGTTGAAGACATC | CGAAGTCTAAGTCTTCTTC | ATCGCTGTGATTACACCACGAC (antisense) | 52 |
| P8 | M774_800 m_11B14a | ACTCGTCTGATTCTTCAC | TTAATACATCTTCCAGTTCCA | AAAGCACAGAACACCATCATG (antisense) | 52 |