Figure 7.
Uptake of DNA in dual-species biofilms of C. albicans and S. mutans. The reporter strain SMPsigXGFP was cultivated for 10 h alone (top panel) or together with C. albicans (middle and bottom panel). DNA labelled with Cy3 was added, and after incubation for 30 min excess DNA was removed by DNAse treatment. See Methods for experimental details. The four rows show (from left to right) the green channel for GPF, the red channel for Cy3, phase contrast and the overlay of red and green channels. Scale bar, 20 μm (top and middle panels) and 5 μm (bottom panel).