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. 2016 Aug 18;7:12444. doi: 10.1038/ncomms12444

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Copyright © 2016, The Author(s)

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(a) Immunohistochemical DAB staining of cochlear sections identifies Slc4a10 expression in the SL fibrocytes of Slc4a10^+/+ and Slc4a10^+/trmb mice. No DAB staining of Slc4a10^trmb/trmb sections is observed at any of the time points tested. Scale bar, 600 μm. Number of cochleae imaged (one per mouse) for each genotype at 2-, 6-, 9- and 12 months of age were: Slc4a10^+/+ n=5, 5, 6, 6; Slc4a10^+/trmb n=7, 4, 5, 6; and Slc4a10^trmb/trmb n=4, 3, 5, 6, respectively. (b) SGN counts in Slc4a10^+/+, Slc4a10^+/trmb and Slc4a10^trmb/trmb mice shows there are no differences across genotype, or with age. Counts averaged from individual mice (N=3) for each genotype at each age. (c) Analysis of the cross-sectional surface area of the SL shows there are no differences across genotypes, or with age. (d) Analysis of the SV show there is a significant reduction in the cross-sectional surface area in Slc4a10^trmb/trmb mice compared with Slc4a10^+/+ and Slc4a10^+/trmb mice, and this is consistent across the ages tested. The SL and SV surface areas are averaged from individual mice for each genotype at 2-, 6-, 9- and 12 months of age: Slc4a10^+/+ n=5, 3, 5, 5; Slc4a10^+/trmb n=7, 5, 8, 5; and Slc4a10^trmb/trmb n=5, 6, 7, 6, respectively. (e,f) Comparison of SL and SV nuclei counts shows there are no differences across genotype, or with age. The nuclei counts are averaged from individual mice for each genotype at 2-, 6-, 9- and 12 months of age: Slc4a10^+/+ n=5, 3, 3, 3; Slc4a10^+/trmb n=5, 4, 4, 3; and Slc4a10^trmb/trmb n=6, 5, 4, 3, respectively. (g) The endocochlear potential is chronically reduced in Slc4a10^trmb/trmb mice compared with controls. Measurements averaged for each genotype at 2-, 9- and 12 months of age: Slc4a10^+/+ n=6, 6, 8; Slc4a10^+/trmb n=10, 12, 11; and Slc4a10^trmb/trmb n=8, 6, 9, respectively. Slc4a10^+/+ (black circles/bars), Slc4a10^+/trmb (grey circles/bars), Slc4a10^trmb/trmb (red circles/bars). Data correspond to mean±s.e.m., and statistical significance determined using two-way ANOVA with Tukey's multiple comparisons test. *P<0.05; **P<0.01; ***P<0.001, ****P<0.0001. ANOVA, analysis of variance.