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. 2016 Aug 19;16:179. doi: 10.1186/s12870-016-0867-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — MiR159 constitutively silences MYB33/65 throughout rosette development. a–c Time-course transcript profiling of the miR159-MYB pathway in rosettes. The relative miRNA and mRNA levels were measured in rosettes approximately every 10 days throughout its development. The miR159 levels were normalized to sno101, the mRNA levels were normalized to CYCLOPHILIN. Values are the mean of three technical replicates with error bars representing the Standard Deviation (SD). Significant differences in values from the previous measurement are indicated with an _*, as determined by the Students T-test. d Time-course GUS-staining assay for rosettes of MIR159b:GUS and mMYB33:GUS transgenic lines. Staining was carried out on ten individual rosettes per time point, at ten-day interval during plant growth; only the first and last staining results are shown