Fig. 1.

Survival curves of 2 days post-fertilization embryos injected through different routes with wild-type Streptococcus pneumoniae D39. a Casper zebrafish embryo at 2 days post - fertilization. Red arrow indicates the hindbrain ventricle infection route, and black arrow indicates the caudal vein infection route. Scale bar, 500 μm. b, c Injection in the b hindbrain ventricle (HBV) or c caudal vein with indicated doses. Hpi hours post injection, CFU colony-forming units. The data represent the mean ± SEM of three individual experiments with 20 embryos in each group