Fig. 2.

Bright-field images with corresponding fluorescent images of 2 day-post-fertilization zebrafish embryos infected by different routes. a, b Lateral view of zebrafish embryos injected in the a hindbrain ventricle or b in the caudal vein. c Non-injected control embryos. Please note that there is usually some background fluorescence observed in the yolk. All embryos were infected with 400 CFU of Streptococcus pneumoniae D39 (HlpA-GFP) and imaged at 48 h post injection. Scale bars, 500 μm