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. 2016 Aug 19;13:188. doi: 10.1186/s12974-016-0655-y

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Pneumococci leave the blood vessels after systemic infection. a, b Confocal microscopy images at maximum projection of Tg(kdrl:mcherry) ^s896 zebrafish embryos injected in the caudal vein (CV) a before formation of the blood-brain barrier (BBB) at 2 days post fertilization (dpf) or b after the formation of the BBB at 4 dpf. Bacteria were localized inside (arrows) and outside (arrow heads) the blood vessels. All embryos were infected with 400 CFU and imaged at 24 h post injection. Scale bars, 50 μm