Additional file 3: Movie S1.
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Time-lapse movie showing the initial innate immune response of Streptococcus pneumoniae-infected zebrafish embryos in the hindbrain ventricle. Dorsal view of Streptococcus pneumoniae-infected double-labelled Tg(mpx:GFP) i114 /Tg (mpeg1:mCherry) gl23 zebrafish embryo (green fluorescent neutrophils, red fluorescent macrophages) via the hindbrain ventricle at 2 days post fertilization. Fifteen minutes after the injection of pneumococci in the hindbrain ventricle, green fluorescent neutrophils migrate in increasing numbers to the site of infection, whereas macrophages were not observed in the hindbrain ventricle. Interval time between frames is 1 min for 2.5 h. Frame rate is 7 frames per second. (MP4 18374 kb)