Table 2.
Reconstruction of individual genomes from a low-complexity pyrene degrading bacterial consortium using the coverage-based clustering module (AB-Cl) for sequence pre-assembly
Abundance class (Bin #) | Number of classified reads | Bin coverage estimate | % Binned reads mapped on reconstructed genome | % Total reads mapped on reconstructed genome | Genomes in given bin (% binned sequences mapped to it) |
---|---|---|---|---|---|
Bin I | 168591494 | 1389 | 82 % | 76,52 % | Bordetella (82 %) |
Bin II | 9445028 | 51 | 97 % | 5,12 % | Mycobacterium (97 %) |
Bin III | 1870808 | 19 | 98 % | 1,04 % | Stenotrophomonas (72 %), Sphingopyxis (22 %), Mycobacterium (3 %) |
Bin IV | 1331276 | 11 | 98 % | 0,83 % | Sphingopyxis (76 %), Stenotrophomonas (19 %), Bordetella (3 %) |
The completeness of the reconstructed genomes was assessed using lineage specific marker genes with CheckM [19], and yielded completeness estimates ranging from 97 to 99 %, with less than 2 % contamination and negligible strain level heterogeneity. Derived from Adam I.K. et al., under review