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. 2016 Aug 19;17:311. doi: 10.1186/s12859-016-1186-3

Table 2.

Reconstruction of individual genomes from a low-complexity pyrene degrading bacterial consortium using the coverage-based clustering module (AB-Cl) for sequence pre-assembly

Abundance class (Bin #) Number of classified reads Bin coverage estimate % Binned reads mapped on reconstructed genome % Total reads mapped on reconstructed genome Genomes in given bin (% binned sequences mapped to it)
Bin I 168591494 1389 82 % 76,52 % Bordetella (82 %)
Bin II 9445028 51 97 % 5,12 % Mycobacterium (97 %)
Bin III 1870808 19 98 % 1,04 % Stenotrophomonas (72 %), Sphingopyxis (22 %), Mycobacterium (3 %)
Bin IV 1331276 11 98 % 0,83 % Sphingopyxis (76 %), Stenotrophomonas (19 %), Bordetella (3 %)

The completeness of the reconstructed genomes was assessed using lineage specific marker genes with CheckM [19], and yielded completeness estimates ranging from 97 to 99 %, with less than 2 % contamination and negligible strain level heterogeneity. Derived from Adam I.K. et al., under review