Table 1.
Bacterial strains and plasmids used in this study
| Strain or Plasmid | Relevant genotype or characteristics | Reference or source |
|---|---|---|
| Strains | ||
| P4 | Agrobacterium tumefaciens. Isolated from tobacco rhizosphere (La Côte Saint André, France) | Mondy et al., 2013 |
| P4cinI | A. tumefaciens; Gmr a, pAtcinI::Gm | This study |
| P4Rif | A. tumefaciens; Rifr | Laboratory collection |
| P4RifcinI | A. tumefaciens; Rifr, Gmr, pAtcinI::Gm | This study |
| P4RifNC | A. tumefaciens; Rifr, Gmr, pAt::Gm | This study |
| P4RifRIX | A. tumefaciens; Rifr, Gmr, pAtΔcinRcinIcinX::Gm | This study |
| C58.00 | A. tumefaciens; Rifr, Smr, cured of the At and Ti plasmids | Laboratory collection |
| NT1(pZLR4) | A. tumefaciens b Gmr, NT1 derived from strain C58, carrying plasmid ZLR4; OC8-HSL biosensor | Cha et al., 1998 ; Pipper et al., 1993 |
| DH5α | Escherichia coli; F, Φ 980dlacZ : M15, recA1, endA1, gyrA96, thi-1, hsdR17(rK–, mK+), supE44,relA1, deoR:(lacZYA–argF)U169 | Hanahan, 1983 |
| Plasmids | ||
| pGEM®-T Easy | Ampr, high copy number | Promega Crop., Madison, WI |
aabbreviations are as follows: Rifr, Smr, Gmr, Ampr, resistant to rifampicin, streptomycin, gentamicin, and ampicillin, respectively
bThis strain is also a derivative of strain C58