Table 2.
PCR primers used in this study
| Targeted gene or region | Primer sequencea | |
|---|---|---|
| P4 cinI | F : | GGCGCAATACCTCAATTGATTG |
| R : | CATCATGGACGTCGTATCGATC | |
| P4 pAt non coding-region (coord. 36817–37590b) | F : | CATCTCGTTGATCGCAACATGGTCGTT |
| R : | GATTTGGATGCTTCAACCGAAGATTTATTTGC | |
| P4 pAt non encoding-region (coord. 36950–37353b) | F : | CCGAAAGTAACATTTGATGCCCCAACTGAATTTT |
| R : | ATAACTGACAGGCATGCGAAAGCCGTC | |
| P4 cinR cinI cinX | F : | TGCTTGTCGTCTGCGCCGTGAGA |
| R : | CTCAAACACCGCTGGAGCGAACTCA | |
| P4 avhB5 RT-qPCR | F: | CCAAGCAGATCGAGAGCAT |
| R: | TTGTTGAGCGAGCCATAAAG | |
| P4 avhB11 RT-qPCR | F: | ACGCTTTCCAAAGCATTGAT |
| R: | CGTGGTTCGGTTGTGAGATA | |
| P4 AGROTU_05920 RT-qPCR | F: | TTGTTGAATGTATGCGGGTT |
| R: | TATGCGCTGTGGAGATATGG | |
| P4 traA RT-qPCR | F: | CGAGCTGTTCCTCACTACAGT |
| R: | TTCAATCTTTCCGACAGCACC | |
| P4 cinR RT-qPCR | F: | CGTCGGAGAGTTCGGTTATT |
| R: | CCTGCAACAAGTCTGTCCC | |
| P4 cinI (internal) RT-qPCR | F: | ATATGGGAAGGCACACGAAT |
| R: | AAAGGAACCCAAACGCTCTA | |
| P4 cinX RT-qPCR | F: | AATATCGGCAGGAGCATTTC |
| R: | GCTCATGCTCAAGGTCAAAG | |
| P4 traG RT-qPCR | F: | GATTTCGATTCGACCCACAT |
| R: | AGCGGGCCTGTATATCTGAG | |
| P4 gyrB RT-qPCR | F: | GATGGCAAGGCCTATGAAAC |
| R: | CACTTCCAGAAGATCGGACA | |
| P4 blcR RT-qPCR | F: | CGACATTCAACGACCATCTC |
| R: | CAGCCGATGTAAACGACTTC |
aall primer sequences are given in the 5′- > 3 orientations. F forward primer, R reverse primer
bcoordinates on pAt P4 with respect to the position of the first and last base amplified by the primer couples