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. 2016 Aug 20;17:31. doi: 10.1186/s12860-016-0109-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Silencing of tyrosine phosphatase PTP-PEST rescues FAK auto-phosphorylation in T47D and TMX2-28 cells. a PTP-PEST siRNA reduces PTP-PEST mRNA in T47D and TMX2-28 cells. T47D and TMX2-28 cells were transfected with either PTP-PEST siRNA or control non-coding siRNA (ctrl) and mRNA levels were assessed by RT-PCR using PTP-PEST-specific primers. GAPDH primers were used an internal control. b WCL of T47D and TMX2-28 clones transfected with control or PTP-PEST siRNAs and treated with PRL (200 ng/ml) for 0 or 15 min were probed for FAK auto-phosphorylation using αpY397-FAK antibody. The expression levels of γtubulin were used as an internal loading control. c Graph represents the densitometric analysis of the bands obtained for pY397-FAK normalized to total FAK