Fig. 3.

Effect of ethanol on GSK3β phosphorylation. a N2a cells grown in a serum free (SF) medium were treated with C3G (5 μM) in the absence or presence of ethanol (400 mg/dl) for specified times. Cell lysates were collected and the expression of phosphorylated GSK3β [p-GSK3β(Ser9) and p-GSK3β(Tyr216)] and total GSK3β was examined with immunoblotting. The experiment was replicated three times. c The relative amounts of p-GSK3β(Ser9) were measured by densitometry and normalized to the expression of total GSK3β. Each data point (mean ± SEM) is the mean of three independent experiments. From (Chen et al. 2009)