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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Mol Microbiol. 2016 Apr 20;100(6):945–953. doi: 10.1111/mmi.13371

Fig. 3.

Fig. 3

Co-expression of gapdh and arsJ confer resistance to arsenate. Overnight cultures of E. coli strain WC3110 or AW3110 bearing either pGAPDH_ArsJ or vector plasmid pUC19 were diluted 100-fold into fresh low phosphate medium containing 0.1 mM As(III), 0.8 mM As(V), 10 μM MAs(III), 0.5 μM PhAs(III), 2 μM Rox(III) or 2 μM Nit(III), as indicated. Expression of the gapdh and arsJ genes was induced with 0.3 mM IPTG. Growth was estimated from the absorption at 600 nm after 24 h at 37°C. Data are the mean±SE (n=3).