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. Author manuscript; available in PMC: 2017 Aug 19.
Published in final edited form as: ACS Chem Biol. 2016 Jun 6;11(8):2105–2111. doi: 10.1021/acschembio.6b00217

Figure 2.

Figure 2

YKL-05-099 displays cell-based activities consistent with SIK inhibition. A) Pre-treatment with the indicated concentrations of YKL-05-099 for 24 hr potentiates IL-10 production by Zymosan A-stimulated BMDCs (mean ± SD; n = 3 from 1 independent experiment; Quantified as % of the response elicited by PGE2). B) Pre-incubation of BMDMs with YKL-05-099 (1 μM) or HG-9-91-01 (1 μM) for 6 hr prior to stimulation with LPS for 30 min reduces p-HDAC5 (Ser259) levels. Ratio of p-HDAC5 / HDAC5 quantified as mean ± SD, n = 2 from 1 independent experiment. C) Effect of pre-treatment with YKL-05-099 (1 μM) for 24 hr on Il10 and Nurr77 transcripts in BMDCs stimulated with LPS for the indicated time points (mean ± SD, n = 6 from 1 independent experiment). D) Pre-incubation of BMDCs with YKL-05-099 (1 μM) for 24 hr modulates inflammatory cytokine production induced by stimulation with Zymosan A for 18 hr (mean ± SD, n = 3 from 1 independent experiment). ***, P < 0.001; **, P < 0.01 using unpaired Student’s t tests. All data are representative of ≥ 2 independent experiments.