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. 2016 May 20;64(3):880–893. doi: 10.1002/hep.28599

Figure 7.

Figure 7

Biliatresone decreases SOX17, and silencing Sox17 phenocopies the biliatresone effect. (A) Sox17 messenger RNA expression in cholangiocytes in 2D culture treated with biliatresone or vehicle (DMSO) for 24 hours. Data are presented as the mean ± standard deviation. *P < 0.05. (B) Western immunoblotting of cholangiocytes in 2D culture treated with biliatresone or vehicle (DMSO) for 24 hours. Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was used as a loading control. (C) Cholangiocyte spheroids treated with siRNA for Sox17 or with scrambled RNA for 24 hours, then stained for ZO1 and with DAPI. Images are representative of three independent experiments with duplicate wells of spheroids. Scale bars: 10 μm. (D) Cholangiocyte spheroids were treated with siRNA for Sox17 for the times indicated and were then immunostained for F‐actin (green) and cellular tubulin (red). Nuclei were stained with DAPI (blue). Images are representative of three independent experiments with duplicate wells of spheroids. Scale bars: 10 μm. (E) GSH levels in cholangiocytes in 2D culture treated with siRNA for Sox17 or scrambled RNA (P = 0.98). Data are presented as the mean ± standard deviation.