Fig. 3.

Effect of the immunization of mastomys with rWbGST on splenocytes proliferation and cytokines profile. a Splenocytes from mastomys administered with rWbGST/Alum were cultured in vitro and re-stimulated with either ConA or rWbGST and the effect on the splenocytes proliferation was checked by MTS assay after 48 h. Each bar represents mean stimulation index (SI) ± SD. The levels of cytokines b IFN-γ and c IL-10, IL-4 were measured in the culture supernatants of splenocytes after 72 h using ELISA. Each bar represents Mean ± SD. n = 5 mastomys per group; * p < 0.05 in comparison with Alum group as analyzed by Mann–Whitney U test