Table 1.
In vitro antibody dependent cellular cytotoxicity induced against B. malayi L3 by pooled sera of mastomys immunized with rWbGST
Number of B. malayi L3 recovered followed by treatment with sera of different groups of mastomys immunized with | |||
---|---|---|---|
Alum | rWbGST | Anti-WbGST Ab depleted sera | |
Live L3 larvae | 18 | 7 | 13 |
19 | 5 | 16 | |
17 | 6 | 16 | |
Dead L3 larvae | 3 | 16 | 4 |
2 | 14 | 3 | |
3 | 15 | 5 | |
% cytotoxicity (Mean ± SD) | 12.93 ± 2.97 | 71.55 ± 2.06* | 21.04 ± 4.55 |
Peritoneal exudate cells (PEC) were incubated with the pooled sera from the mastomys administered with rWbGST/Alum/anti-WbGST Ab depleted sera and L3 larvae in vitro. The total live and dead worms were counted in each well after 48 h. The data shown is the number of larvae recovered from three different sets of experiments
* p = 0.035 in comparison with Alum and anti-WbGST Ab depleted groups as analysed by Kruskal–Wallis test followed by Dunn’s post hoc test