Fig. 4.

DV infection causes bone tissue inflammation and disturbance of bone homeostasis in STAT1-deficient mice. a Representative ¹⁸F-FDG PET/CT imaging of the STAT1-deficient mice at days 3, 5, and 7 after DV infection. Increased ¹⁸F-FDG uptake in the left sacroiliac junction (arrow) was observed from day 3 postinfection and subsided at day 7. Normal variations of tracer uptake in brown fat of the upper back region (arrow head) and paraspinal muscles (double arrow head) and tracer accumulation in the urinary bladder (asterisk) were noted. b ^99mTc-MDP SPECT/CT bone scan at day 5 postinfection showed slightly increased bony uptake of radiotracer in the upper part of left sacroiliac junction (arrow), and more intense uptake was detected at day 7. Tracer was accumulated in the urinary bladder (asterisk). Data from a, b were collected from five independent experiments. c Serum of DV-infected mice were harvested at indicated days after DV inoculation and C-telopeptide of type I collagen (CTX-1) were determined by RatLaps ELISA kit. Data were collected and expressed as mean ± SEM. ANOVA tests were performed