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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Immunol. 2016 Jul 20;197(5):1754–1761. doi: 10.4049/jimmunol.1600641

Figure 1.

Figure 1

3×104 Thy1.1+ WT or CD45.2+ Bim−/ − OT-I cells were adoptively transferred into naïve Thy1.2+ or CD45.1+ B6 mice, respectively, and immunized with 5×105 DC-Ova followed by Rat Ig, 0.2μg or 1.5μg IL-2c on D4–6 post-DC immunization. (A) Representative flow plots depicting OT-I cell frequencies in the blood at D7 and D90 post-DC immunization. (B) Kinetics graph of total number of WT OT-I cells quantified per mL of blood longitudinally across 90 days in all treatment groups. (C) Summary bar graph (mean ± SEM) of total number of OT-I cells harvested from the spleen at D90 post-DC immunization. (D) Same as (A) except frequency of Bim−/ − OT-I cells. (E) Same as (B) except total number of Bim−/ − OT-I cells in the blood. (F) Immunoblot of BimEL and BimL isoforms in WT OT-I cells harvested from spleen at D6 post-DC immunization across treatment groups (top). Immunoblot of β-actin protein expression as loading control (bottom). Data are representative of two experiment with at least n=5 mice/group/experiment. * = p<0.05; ** = p<0.005; *** = p<0.0005; ns, not significant.