Table 5.
Validation of whole-genome gene expression with QRT-PCR.
| Gene name |
Protein | Function | Fold change (p value)* | |
|---|---|---|---|---|
| Microarray | QRT-PCR | |||
| Nup35 | Nucleoporin 35 | Component of the nuclear pore complex | 1.48 ± 0.10 (0.019) | 1.07 ± 0.07 (0.445) |
| Zbtb16 | Zinc finger protein 145 | Transcription factor | 1.41 ± 0.19 (0.041) | 2.46 ± 0.43 (0.003) |
| Entpd4 | Ectonucleoside triphosphate diphosphohydrolase 4 | Hydrolase activity | 1.35 ± 0.05 (0.0004) | 1.27 ± 0.11 (0.042) |
| Npy | Pro-neuropeptide Y | Involved in feeding and secretion of gonadotrophin-releasing hormone |
1.29 ± 0.12 (0.019) | 1.11 ± 0.08 (0.253) |
| Mpp4 | MAGUK p55 subfamily member 4 | Synaptic function | 1.25 ± 0.03 (0.00003) | −1.04 ± 0.05 (0.206) |
| Creld2 | Cysteine-rich with EGF-like domains 2 | Calcium ion binding | 1.23 ± 0.08 (0.008) | 1.27 ± 0.08 (0.045) |
| Psat1 | Phosphoserine aminotransferase | Phosphoserine aminotransferase activity | −1.27 ± 0.04 (0.002) | −1.11 ± 0.07 (0.066) |
| Psat1 | Phosphoserine aminotransferase | Phosphoserine aminotransferase activity | −1.27 ± 0.04 (0.002) | −1.11 ± 0.07 (0.066) |
| Nkain2 | Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 2 |
Regulation of sodium ion transport | −1.27 ± 0.04 (0.004) | −1.52 ± 0.14 (0.018) |
| Zfhx4 | Zinc finger homeobox protein 4 | Transcriptional regulation | −1.34 ± 0.06 (0.013) | −1.82 ± 0.08 (0.011) |
| Nr4a1 | Nuclear receptor subfamily 4 group A member 1 | Orphan nuclear receptor | −1.36 ± 0.05 (0.031) | −1.52 ± 0.05 (0.008) |
| Opalin | Oligodendrocytic myelin paranodal and inner loop protein | Integral component of membrane | −1.37 ± 0.07 (0.010) | −1.45 ± 0.06 (0.015) |
| Nr4a2 | Nuclear receptor subfamily 4 group A member 2 | Transcriptional regulator | −1.60 ± 0.07 (0.005) | −1.75 ± 0.06 (0.006) |
*
Means ± SEM (p value).