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. 2016 Aug 22;7:113. doi: 10.3389/fendo.2016.00113

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Copyright © 2016 Salmerón, Riera-Heredia, Gutiérrez, Navarro and Capilla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression profile of lipid metabolism-related genes in gilthead sea bream preadipocytes during adipogenesis. The mRNA levels of lpl (A), fas (B), g6pdh (C), hsl (D), gapdh1 (E), and gapdh2 (F) were measured by quantitative real-time PCR and normalized to ef1α and rps18. Samples were taken from preadipocytes (days −4 and 0 of culture) and differentiated adipocytes (days 4, 8, and 12 of culture). Values are means ± SEM, n = 4–5. Bars with different letters are significantly different (P < 0.05). lpl, lipoprotein lipase; fas, fatty acid synthase; g6pdh, glucose-6-phosphate dehydrogenase; hsl, hormone-sensitive lipase; gapdh1 and 2, glyceraldehyde 3-phosphate dehydrogenase 1 and 2, respectively; ef1α, elongation factor 1 alpha; rps18, ribosomal protein S18.