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Western-blot analysis of B. cinerea using antibodies histone H3 or trimethylated H3K9. In each case, 100 μg total proteins from wild-type B05.10, ΔBcDIM5-21, or BcDIM5-C1 strains were fractionated by SDS–PAGE (12%) and analyzed using the indicated antibodies. The monoclonal anti-Histone H3 was used as internal loading reference.
