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. 2016 Aug 8;2016:5108489. doi: 10.1155/2016/5108489

Figure 2.

Figure 2

Knockdown of miR-132 and p250GAP in cultured neurons. (a) Cells were transfected with ant-132 at DIV7 and observed using a fluorescence microscope 48 h after transfection. Transfection efficiency was measured by RFP fluorescence. (b) Cells were transfected with LV-shp250GAP at DIV7 and observed 72 h after transfection. Transfection efficiency was measured by GFP fluorescence. Scale bar is 50 µm. (c, d) Measurement of knocked down expression in transfected neurons was performed by qRT-PCR for miR-132 and by WB for p250GAP. (e) Expression level of p250GAP 72 h after ant-132 treatment in cultured epileptic hippocampal neurons. ∗∗ p < 0.01 (n = 10; the data are representative of 5 experiments).