Figure 2.

Accumulation of antigen-specific memory B cells and CD4 positive T cells in artTLOs. artTLOs were constructed in Balb/c mice, which had been preimmunized with 100 μg NP-hapten conjugated ovalbumin (NP-OVA) in alum as described in the Section “Materials and Methods.” After 3 weeks, artTLO were formed in the subcapsular space of kidney. artTLO and spleen were removed from recipients for flow cytometer analysis. Flow cytometer profiles of artTLO and spleen cells of recipient mice are shown. Note that IgG1 class anti-NP antibody-forming cells as well as memory B cells and effector memory T cells are remarkably enriched in artTLOs, compared to the recipient spleen cells. Representative data are shown from FACS analysis of 10 individual artTLO. Data were highly reproducible.