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. 2016 Aug 22;6:31848. doi: 10.1038/srep31848

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Copyright © 2016, The Author(s)

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(A) SDS-PAGE analysis of tatsi from both male and female showed the abundant presence of low molecular weight bands mainly in the crude extracts from the tarsi of male C. japonicus. (B) A crude extract of male tarsi was separated on a native gel, and two fast migrating bands were selected for N-terminal sequencing. mT, male tarsi; fT, female tarsi. Molecular mass markers (M) are (from the top): 97 kDa (phosphorylase B), 66 kDa (BSA), 45 kDa (ovalbumin), 29 kDa (carbonic anhydrase), 20 kDa (trypsin inhibitor) and 14 kDa (α-lactalbumin).