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. 2016 Aug 22;6:31848. doi: 10.1038/srep31848

Figure 4. SDS-PAGE and Western blot of crude extracts from different parts of the body of adult C.japonicus.

Figure 4

(A) SDS-PAGE analysis of crude extracts from different tissues of male and female C. japonicas, showed the presence of low molecular weight bands mainly in the male tarsi sample. Western blot membrane after reaction with polyclonal antiserum against CjapOBP1 (B) or CjapOBP2 (C) and peroxidase-conjugated second antibody. Ant, antennae; Tar, tarsi; Pal, palpi; AG, accessory gland; and Tes, testis. Molecular mass markers (M) are (from the top): 97 kDa (phosphorylase B), 66 kDa (BSA), 45 kDa (ovalbumin), 29 kDa (carbonic anhydrase), 20 kDa (trypsin inhibitor) and 14 kDa (α-lactalbumin).