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. 2016 Aug 22;6:31758. doi: 10.1038/srep31758

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Hs68 cells at passage numbers 30, 58, and 65 (P30, P58, and P65) were subjected to SA-β-Gal staining (a), qPCR (b), and immunoblot analysis (c). (a) The percentage of SA-β-Gal-positive cells (left) and representative images (right) are shown. Bars, 50 μm. (b) Relative mRNA levels of indicated genes are shown. (c) The protein levels relative to the γ-tubulin level were quantified using NIH ImageJ software and are indicated at the bottom of each lane. The uncropped images are shown in Supplementary Fig. 8. Data are mean ± SD. Statistical significance is shown using the Student’s t-test analysis (n = 3); *P < 0.05; **P < 0.01.