Table 1. Relative mRNA levels of selected genes in HepG2 cells treated with 10 and 100 μM etoposide for 30 h.
| Gene | Microarray results (Fold change) | qPCR results (Fold change ± SD) | Molecular class | |||
|---|---|---|---|---|---|---|
| 10 μM/DMSO | 100 μM/DMSO | 10 μM/100 μM | 10 μM/DMSO | 100 μM/DMSO | ||
| PVRL4 | 32.09 | 14.46 | 2.22 | 22.30 ± 0.74 | 15.37 ± 0.65 | Adhesion molecule |
| GPR172B | 19.25 | 9.57 | 2.01 | 45.13 ± 1.24 | 23.71 ± 1.39 | G protein coupled receptor |
| DAO | 13.55 | 6.62 | 2.05 | 15.69 ± 2.62 | 9.46 ± 0.93 | Oxidase |
| CCDC74B | 9.47 | 2.81 | 3.37 | 1.56 ± 0.13 | 1.58 ± 0.10 | Unclassified |
| LOXL4 | 8.66 | 3.39 | 2.55 | 5.41 ± 0.32 | 2.95 ± 0.38 | Oxidase |
| EVL | 8.65 | 4.11 | 2.10 | 16.36 ± 1.39 | 3.65 ± 0.08 | Cytoskeletal protein |
| PRODH | 8.42 | 3.71 | 2.27 | 8.62 ± 0.57 | 3.66 ± 0.46 | Dehydrogenase |
| E2F7 | 7.94 | 1.21 | 6.56 | 7.59 ± 0.74 | 1.63 ± 0.02 | Transcription regulation |
| LY6D | 7.45 | 1.58 | 4.72 | 6.66 ± 0.23 | 1.80 ± 0.06 | Adhesion molecule |
| IGFBP2 | 7.30 | 3.48 | 2.10 | 33.60 ± 1.54 | 10.75 ± 0.23 | Secreted polypeptide |
| CRABP2 | 7.16 | 1.89 | 3.79 | 1.11 ± 0.14 | 1.44 ± 0.16 | Transcription regulation |
| EPN3 | 6.41 | 3.07 | 2.09 | 6.98 ± 0.47 | 3.17 ± 0.32 | Unclassified |
| APOBEC3B | 4.99 | 1.22 | 4.09 | 5.96 ± 0.24 | 1.57 ± 0.16 | RNA binding protein |
| IER5 | 3.85 | 1.80 | 2.14 | 3.66 ± 0.20 | 1.97 ± 0.05 | Transcription regulation |
| ANGPTL2 | 3.63 | 1.76 | 2.06 | 3.00 ± 0.38 | 1.88 ± 0.13 | Secreted polypeptide |
| SLC48A1 | 3.50 | 1.37 | 2.55 | 3.00 ± 0.11 | 1.61 ± 0.04 | Transport/cargo protein |
| WBSCR27 | 3.41 | 1.61 | 2.12 | 2.94 ± 0.07 | 1.79 ± 0.35 | Unclassified |
| E2F2 | 3.33 | 0.27 | 12.33 | 2.33 ± 0.15 | 0.33 ± 0.09 | Transcription regulation |
| NXPH4 | 3.09 | 1.54 | 2.01 | 1.11 ± 1.03 | 1.06 ± 0.26 | Secreted polypeptide |
| PPM1D | 3.08 | 1.47 | 2.10 | 3.14 ± 0.13 | 1.80 ± 0.09 | Serine/threonine phosphatase |
| p21 | 7.94 | 7.07 | 1.12 | 6.40 ± 0.54 | 5.43 ± 0.27 | Cell Cycle control |
| BTG2 | 10.13 | 6.82 | 1.49 | 7.92 ± 0.81 | 7.17 ± 0.68 | Transcription regulation |
| SULF2 | 19.37 | 9.78 | 1.98 | 10.60 ± 0.08 | 6.87 ± 0.09 | Sulfatase |
Twenty genes that were differentially upregulated at the low dose of etoposide were selected by microarray analysis. Differential upregulation of genes in bold type were confirmed by qPCR.