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. 2016 Aug 22;6:31409. doi: 10.1038/srep31409

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Copyright © 2016, The Author(s)

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(a) Gastrocnemius muscles were collected at 2, 5, 10 and 14 d after burn injury. Western blot analysis of MG53 protein levels with MG53 antibodies. Data were normalized to the mean control value as determined by the sham-injured group. *P < 0.05 vs. Control; n = 3. (b) Immunohistochemical analysis of MG53 protein in the gastrocnemius muscle of sham control (C) and burn injured (B) (Post Burn Day 14) mice (Scale bar, 200 μm). (c) Gastrocnemius muscles were collected at 2, 5, 10 and 14 d after burn injury. Western blot analysis of MG53 dimer levels with MG53 antibodies in the absence of dithiothreitol (DTT). Data were then normalized to the mean control value as determined by the sham-injured group. *P < 0.05 vs. Control; n = 3.