Figure 2. Changes in the phosphorylation levels of known and new SnRK1 targets.

(a) Classical targets are NR, F2KP, SPS and TPS. New targets are bZIP63 and two uncharacterized transcription factors. Relative phosphopeptide abundances were normalized to the corresponding wild type (ko to Col-0 and OEs to Ler). Asteriks indicate significant differences in ttest between mutant and the wild type (*p < 0.05, **p < 0.01). The quantified SnRK1 consensus motif containing peptides are shown above the charts. The phosphorylated residue is shown in red, basic residue at the −3 or −4 position are indicated in blue and in green are shown the hydrophobic residues at the −5 and +4 positions. Tryptic peptides measured by LC-MS are in bold.