Table 2. Microarray analysis of hepatic gene expression profile based on genes identified in human NAFLD.
| Probe ID | Gene symbol | Gene name | HFD vs. Chow | HFD + Rosi vs. HFD | ||||
|---|---|---|---|---|---|---|---|---|
| Fold-Change | p-value | Fold-Change | p-value | |||||
| ILMN_2635229 | Thbs2 | thrombospondin 2 | 1,740 | ↑ | 9,47E-06 | 0,650 | ↓ | 4,62E-04 |
| ILMN_2764588 | Igfbp7 | insulin-like growth factor binding protein 7 | 1,465 | ↑ | 1,79E-05 | 0,834 | ↓ | 3,52E-02 |
| ILMN_1217309 | Tax1bp3 | Tax1 (human T-cell leukemia virus type I) binding protein 3 | 1,360 | ↑ | 7,45E-04 | 0,826 | ↓ | 3,28E-02 |
| ILMN_2866901 | Efemp2 | epidermal growth factor-containing fibulin-like extracellular matrix protein 2 | 1,556 | ↑ | 7,62E-04 | 0,751 | ↓ | 2,70E-02 |
| ILMN_2636424 | Itgbl1 | integrin, beta-like 1 | 1,528 | ↑ | 1,11E-03 | 0,905 | 4,33E-01 | |
| ILMN_2746556 | Dkk3 | dickkopf homolog 3 (Xenopus laevis) | 1,402 | ↑ | 1,16E-03 | 0,744 | ↓ | 4,27E-03 |
| ILMN_1258629 | Col3a1 | collagen, type III, alpha 1 | 1,887 | ↑ | 1,61E-03 | 0,746 | 1,39E-01 | |
| ILMN_2939138 | Bicc1 | bicaudal C homolog 1 (Drosophila) | 1,460 | ↑ | 2,69E-03 | 0,666 | ↓ | 1,28E-03 |
| ILMN_2746086 | Tax1bp3 | Tax1 (human T-cell leukemia virus type I) binding protein 3 | 1,334 | ↑ | 2,94E-03 | 0,787 | ↓ | 1,27E-02 |
| ILMN_2980663 | Aqp1 | aquaporin 1 | 0,812 | ↓ | 7,68E-03 | 1,158 | ↑ | 5,74E-02 |
| ILMN_2606210 | Dpt | dermatopontin | 1,459 | ↑ | 1,08E-02 | 0,714 | ↓ | 2,26E-02 |
| ILMN_3007428 | Sox9 | SRY-box containing gene 9 | 0,694 | ↓ | 1,11E-02 | 1,245 | 1,23E-01 | |
| ILMN_2831656 | Epha3 | Eph receptor A3 | 1,334 | ↑ | 1,76E-02 | 0,901 | 3,84E-01 | |
| ILMN_2687872 | Col1a1 | collagen, type I, alpha 1 | 1,471 | ↑ | 3,99E-02 | 0,921 | 6,58E-01 | |
| ILMN_2747959 | Dcn | decorin | 1,151 | ↑ | 4,22E-02 | 0,874 | ↓ | 5,21E-02 |
| ILMN_2591027 | Col14a1 | collagen, type XIV, alpha 1 | 1,176 | ↑ | 4,74E-02 | 0,820 | ↓ | 1,61E-02 |
| ILMN_1223552 | Fbn1 | fibrillin 1 | 1,181 | 6,25E-02 | 0,885 | 1,69E-01 | ||
| ILMN_1233545 | Lbh | limb-bud and heart | 0,782 | 6,40E-02 | 1,089 | 5,19E-01 | ||
| ILMN_2669189 | Lima1 | LIM domain and actin binding 1 | 1,226 | 8,23E-02 | 0,956 | 6,98E-01 | ||
| ILMN_1253806 | Col1a2 | collagen, type I, alpha 2 | 1,278 | 8,24E-02 | 0,837 | 2,08E-01 | ||
| ILMN_2852957 | Dkk3 | dickkopf homolog 3 (Xenopus laevis) | 1,184 | 8,82E-02 | 0,941 | 5,38E-01 | ||
| ILMN_1214954 | Cldn10 | claudin 10 | 0,836 | 1,39E-01 | 1,143 | 2,68E-01 | ||
| ILMN_1228374 | Lima1 | LIM domain and actin binding 1 | 1,190 | 1,48E-01 | 0,916 | 4,66E-01 | ||
| ILMN_2980661 | Aqp1 | aquaporin 1 | 0,895 | 1,89E-01 | 1,124 | 1,65E-01 | ||
| ILMN_1226183 | Antxr1 | anthrax toxin receptor 1 | 1,211 | 1,91E-01 | 0,815 | 1,63E-01 | ||
| ILMN_2848305 | Pnma1 | paraneoplastic antigen MA1 | 1,144 | 1,96E-01 | 0,993 | 9,43E-01 | ||
| ILMN_2666018 | Mgp | matrix Gla protein | 1,162 | 1,99E-01 | 0,914 | 4,39E-01 | ||
| ILMN_2816180 | Lbh | limb-bud and heart | 1,137 | 2,24E-01 | 0,902 | 3,29E-01 | ||
| ILMN_1257077 | Jag1 | jagged 1 | 1,160 | 2,33E-01 | 0,941 | 6,22E-01 | ||
| ILMN_2734683 | Fstl1 | follistatin-like 1 | 1,119 | 2,71E-01 | 0,949 | 6,11E-01 | ||
| ILMN_2596346 | Dcn | decorin | 1,102 | 3,26E-01 | 0,834 | 6,82E-02 | ||
| ILMN_2597515 | Ehf | ets homologous factor | 1,147 | 3,35E-01 | 1,055 | 7,05E-01 | ||
| ILMN_3001540 | Lum | lumican | 1,101 | 4,53E-01 | 0,817 | 1,17E-01 | ||
| ILMN_1227817 | Ank3 | ankyrin 3, epithelial | 1,109 | 4,57E-01 | 0,940 | 6,58E-01 | ||
| ILMN_2769479 | Lama2 | laminin, alpha 2 | 1,116 | 4,87E-01 | 0,968 | 8,38E-01 | ||
| ILMN_2893417 | Sox4 | SRY-box containing gene 4 | 0,929 | 5,57E-01 | 0,965 | 7,75E-01 | ||
| ILMN_1223963 | Ank3 | ankyrin 3, epithelial | 1,081 | 5,62E-01 | 0,835 | 1,83E-01 | ||
| ILMN_2836637 | Glt8d2 | glycosyltransferase 8 domain containing 2 | 1,078 | 5,91E-01 | 1,229 | 1,45E-01 | ||
| ILMN_1249021 | Bcl2 | B-cell leukemia/lymphoma 2 | 1,058 | 5,99E-01 | 0,937 | 5,50E-01 | ||
| ILMN_1229643 | Antxr1 | anthrax toxin receptor 1 | 1,066 | 6,02E-01 | 0,779 | ↓ | 4,37E-02 | |
| ILMN_2620563 | Nexn | nexilin | 1,081 | 6,07E-01 | 0,771 | 8,88E-02 | ||
| ILMN_1238000 | Srpx | sushi-repeat-containing protein | 1,056 | 6,75E-01 | 1,095 | 4,83E-01 | ||
| ILMN_2621643 | Col4a1 | collagen, type IV, alpha 1 | 1,044 | 7,12E-01 | 1,125 | 3,12E-01 | ||
| ILMN_2629486 | Srpx | sushi-repeat-containing protein | 0,958 | 7,70E-01 | 0,832 | 2,12E-01 | ||
| ILMN_2686036 | Tax1bp3 | Tax1 (human T-cell leukemia virus type I) binding protein 3 | 1,030 | 8,04E-01 | 1,004 | 9,72E-01 | ||
| ILMN_2701712 | Plcxd3 | phosphatidylinositol-specific phospholipase C, X domain containing 3 | 0,982 | 8,80E-01 | 0,943 | 6,22E-01 | ||
| ILMN_2629804 | Epha3 | Eph receptor A3 | 0,987 | 9,04E-01 | 1,100 | 3,67E-01 | ||
The table lists the genes that were recently reported to be associated with NAFLD severity in humans19. HFD feeding of LDLr−/− mice resulted in a significant effect on 16 genes compared to chow (arrows indicate significant up- (↑) or downregulation (↓)). Rosiglitazone counteracted the effect of a HFD as shown by the comparison of HFD + Rosi vs. HFD.