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. 2016 Aug 22;6:31125. doi: 10.1038/srep31125

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Chromatin prepared from L267 cells was immunoprecipitated with antibodies directed against different RNAPIII transcription initiation factors or with an IgG as control. Purified DNA was then amplified with oligonucleotide primers hybridizing either to the 5′LTR, the miRNA cluster or the 3′LTR/host junction regions (a), either to the type 1 RNAPIII promoter of the 5S rDNA gene (b), the type 2 RNAPIII promoter of the tRNA^asp gene (c) or to the type 3 RNAPIII promoter of the 7SK gene (d). Results are presented as histograms indicating percentages of immunoprecipitated DNA compared to the input DNA (% IP/INP). Data are the means ± SEM from one representative of at least three independent experiments.