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. 2016 Aug 22;6:31125. doi: 10.1038/srep31125

Figure 7. The RNAPII promoter in the 3′LTR is transcriptionally active.

Figure 7

Raji cells were transiently co-transfected with (a) 400 ng of pREP-luc, pREP-LTR(wt)-S-luc or pREP-LTR(wt)-AS-luc constructs, increasing amounts of an expression vector for Tax (50 or 150 ng of plasmid DNA) and 20 ng of pRL-TK or with (b) 400 ng of pREP-luc, pREP-LTR(wt) -AS-luc or pREP-LTR(MTE/DPE*)-AS-luc, pREP-LTR(BRE*)-AS-luc or pREP-LTR(MTE/DPE* & BRE*)-AS-luc mutated constructs and 20 ng of pRL-TK. Forty-eight hours after transfection, cells were collected, lysed and both firefly and renilla luciferase activities were measured. Results are presented as histograms indicating relative luciferase activities compared to the pREP-luc for wich a value of 1 was assigned. Data are the mean ± SEM from one representative of at least three independent experiments.