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. 2016 Aug 22;6:31769. doi: 10.1038/srep31769

Figure 6. Osteogenic differentiation.

Figure 6

Alkaline phosphatase (a) and matrix mineralization (b) semi-quantitative assay; (c) Expression of osteogenic-related differentiation genes (ALP, OCN, OPN and BSP) were measured by real-time PCR. (d) Alizarin Red S staining at 10-fold magnification. (e) Expression of OCN was detected by immunofluorescent staining after 14 days culture.