Figure 6. Osteogenic differentiation.
Alkaline phosphatase (a) and matrix mineralization (b) semi-quantitative assay; (c) Expression of osteogenic-related differentiation genes (ALP, OCN, OPN and BSP) were measured by real-time PCR. (d) Alizarin Red S staining at 10-fold magnification. (e) Expression of OCN was detected by immunofluorescent staining after 14 days culture.