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. 2016 Aug 22;6:31782. doi: 10.1038/srep31782

Figure 1. Adaptation of tracrRNA sequence to improve CRISPR–Cas9 knock-out screening.

Figure 1

(A) The sequence of the original tracrRNA from the fusion molecule18 and found in pLentiCRISPRv213. (B) The adapted tracrRNA providing apparent increased residency time of the Cas9 and target DNA12.